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rich r2a broth  (Teknova)


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    Structured Review

    Teknova rich r2a broth

    Rich R2a Broth, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r2a+broth/R2A+Broth/bio_rxiv__64898__2025__12__06__692658-132-10-16
    Average 94 stars, based on 42 article reviews
    rich r2a broth - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Leveraging CryoEM and AI-Driven Morphological Feature Analysis for Insights on Bacterial Structures"

    Article Title: Leveraging CryoEM and AI-Driven Morphological Feature Analysis for Insights on Bacterial Structures

    Journal: bioRxiv

    doi: 10.64898/2025.12.06.692658


    Figure Legend Snippet:

    Techniques Used:

    Quantitative analysis using the bacterial cell envelope thickness tool. ( a ) Radial plots of Pantoea sp. YR343 envelope thickness in R2A, MOPS + glucose, and MOPS + succinate media. The 0° position marks pole 1, followed counterclockwise by side 1 (90°), pole 2 (180°), and side 2 (270°). ( b ) Violin plots showing global thickness distributions across media types. ( c ) Correlation between AI- and manual-based thickness measurements (n=50).
    Figure Legend Snippet: Quantitative analysis using the bacterial cell envelope thickness tool. ( a ) Radial plots of Pantoea sp. YR343 envelope thickness in R2A, MOPS + glucose, and MOPS + succinate media. The 0° position marks pole 1, followed counterclockwise by side 1 (90°), pole 2 (180°), and side 2 (270°). ( b ) Violin plots showing global thickness distributions across media types. ( c ) Correlation between AI- and manual-based thickness measurements (n=50).

    Techniques Used:

    Quantitative analysis using the FOV tool. ( a ) Low-magnification grid-square cryoEM image of Pantoea sp. YR343 in R2A at 260 × showing bacteria (green circles/arrows) and ice crystals (yellow arrows) in vitreous ice. Scale bar: 10 µm. ( b ) Search-map montage at 3.8 k× showing higher-resolution views of bacteria and cubic/hexagonal ice. ( c ) AI-derived bacterial counts per grid square across media. ( d ) Correlation between AI- and manual-based counts across grid squares (R²=0.97).
    Figure Legend Snippet: Quantitative analysis using the FOV tool. ( a ) Low-magnification grid-square cryoEM image of Pantoea sp. YR343 in R2A at 260 × showing bacteria (green circles/arrows) and ice crystals (yellow arrows) in vitreous ice. Scale bar: 10 µm. ( b ) Search-map montage at 3.8 k× showing higher-resolution views of bacteria and cubic/hexagonal ice. ( c ) AI-derived bacterial counts per grid square across media. ( d ) Correlation between AI- and manual-based counts across grid squares (R²=0.97).

    Techniques Used: Bacteria, Derivative Assay

    Related Articles

    other:

    Article Title: Diisocyanates influence models of atopic dermatitis through direct activation of TRPA1
    Article Snippet: Roseomonas was grown in R2A broth (Teknova; Hollister, CA) or on R2A agar (Remel; San Diego, CA) at 32°C.

    Article Title: Antimony Compounds Associate with Atopic Dermatitis and Influence Models of Itch and Dysbiosis.
    Article Snippet: Isolates of R. mucosa were selected as previously described.17−19 Roseomonas was grown in R2A broth (Teknova; Hollister, CA) or on R2A agar (Remel; San Diego, CA) at 32 °C.

    Article Title: Antimony Compounds Associate with Atopic Dermatitis and Influence Models of Itch and Dysbiosis
    Article Snippet: 17 – 19 Roseomonas was grown in R2A broth (Teknova; Hollister, CA) or on R2A agar (Remel; San Diego, CA) at 32 °C.

    Article Title: Exposure to isocyanates predicts atopic dermatitis prevalence and disrupts therapeutic pathways in commensal bacteria
    Article Snippet: Roseomonas was grown in R2A broth (Teknova, Hollister, CA) or on R2A agar (Remel, San Diego, CA) at 32°C.

    Article Title: Engineered biocatalysts for non-sterile cultivation and solvent-free biolipid bioextraction
    Article Snippet: R2A broth was purchased from Teknova (Hollister, CA, USA).

    Expressing:

    Article Title: Controlling matric potential in microfluidics to examine microbial dynamics in unsaturated porous media
    Article Snippet: .. Pantoea sp. YR343 cells, expressing green fluorescent protein (GFP) (Bible et al., 2016, 2021), were grown overnight in 0.2X diluted R2A broth (TEKnova. ..

    Cell Culture:

    Article Title: Therapeutic responses to Roseomonas mucosa in atopic dermatitis may involve lipid-mediated TNF-related epithelial repair
    Article Snippet: .. For select experiments, R. mucosa was cultured in R2A broth (Teknova, Hollister, CA) containing 1% lipase (v/v) from Candida spp. (Sigma-Aldrich). ..



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    Image Search Results


    Journal: bioRxiv

    Article Title: Leveraging CryoEM and AI-Driven Morphological Feature Analysis for Insights on Bacterial Structures

    doi: 10.64898/2025.12.06.692658

    Figure Lengend Snippet:

    Article Snippet: Single colonies were used to inoculate liquid cultures in either rich R2A broth (R2A Broth Premix, TEKnova, Inc.), minimal MOPS medium supplemented with 0.4% glucose (MOPS + glucose) or minimal MOPS medium supplemented with 0.4 succinate (MOPS + succinate).

    Techniques:

    Quantitative analysis using the bacterial cell envelope thickness tool. ( a ) Radial plots of Pantoea sp. YR343 envelope thickness in R2A, MOPS + glucose, and MOPS + succinate media. The 0° position marks pole 1, followed counterclockwise by side 1 (90°), pole 2 (180°), and side 2 (270°). ( b ) Violin plots showing global thickness distributions across media types. ( c ) Correlation between AI- and manual-based thickness measurements (n=50).

    Journal: bioRxiv

    Article Title: Leveraging CryoEM and AI-Driven Morphological Feature Analysis for Insights on Bacterial Structures

    doi: 10.64898/2025.12.06.692658

    Figure Lengend Snippet: Quantitative analysis using the bacterial cell envelope thickness tool. ( a ) Radial plots of Pantoea sp. YR343 envelope thickness in R2A, MOPS + glucose, and MOPS + succinate media. The 0° position marks pole 1, followed counterclockwise by side 1 (90°), pole 2 (180°), and side 2 (270°). ( b ) Violin plots showing global thickness distributions across media types. ( c ) Correlation between AI- and manual-based thickness measurements (n=50).

    Article Snippet: Single colonies were used to inoculate liquid cultures in either rich R2A broth (R2A Broth Premix, TEKnova, Inc.), minimal MOPS medium supplemented with 0.4% glucose (MOPS + glucose) or minimal MOPS medium supplemented with 0.4 succinate (MOPS + succinate).

    Techniques:

    Quantitative analysis using the FOV tool. ( a ) Low-magnification grid-square cryoEM image of Pantoea sp. YR343 in R2A at 260 × showing bacteria (green circles/arrows) and ice crystals (yellow arrows) in vitreous ice. Scale bar: 10 µm. ( b ) Search-map montage at 3.8 k× showing higher-resolution views of bacteria and cubic/hexagonal ice. ( c ) AI-derived bacterial counts per grid square across media. ( d ) Correlation between AI- and manual-based counts across grid squares (R²=0.97).

    Journal: bioRxiv

    Article Title: Leveraging CryoEM and AI-Driven Morphological Feature Analysis for Insights on Bacterial Structures

    doi: 10.64898/2025.12.06.692658

    Figure Lengend Snippet: Quantitative analysis using the FOV tool. ( a ) Low-magnification grid-square cryoEM image of Pantoea sp. YR343 in R2A at 260 × showing bacteria (green circles/arrows) and ice crystals (yellow arrows) in vitreous ice. Scale bar: 10 µm. ( b ) Search-map montage at 3.8 k× showing higher-resolution views of bacteria and cubic/hexagonal ice. ( c ) AI-derived bacterial counts per grid square across media. ( d ) Correlation between AI- and manual-based counts across grid squares (R²=0.97).

    Article Snippet: Single colonies were used to inoculate liquid cultures in either rich R2A broth (R2A Broth Premix, TEKnova, Inc.), minimal MOPS medium supplemented with 0.4% glucose (MOPS + glucose) or minimal MOPS medium supplemented with 0.4 succinate (MOPS + succinate).

    Techniques: Bacteria, Derivative Assay

    A | After pre-culturing each of the 16 species of the community for one growth cycle, we adjusted optical density of each culture, pooled them at equal volume and inoculated 96 resource environments, with each condition replicated 8 times. Each culture was serially transferred for 16 days, with 1% of the culture transferred every 48 h. B | At each transfer, we measured OD 600 of each community. In addition, we conducted 16S rRNA sequencing of all communities at the end-point and measured growth traits of each of the dominant species ( A. caviae, P. chlororaphis, C. koseri ) in monoculture in all carbon levels in the absence of R2A. Alt text. Two-panel schematic representing the study set-up. Panel A describes parameters of the serial passaging experiment. Panel B explains analytical procedures of the community and single-species experiments.

    Journal: bioRxiv

    Article Title: Ecological tristability driven by total carbon availability over resource complexity in a synthetic microbial community

    doi: 10.64898/2026.02.26.708221

    Figure Lengend Snippet: A | After pre-culturing each of the 16 species of the community for one growth cycle, we adjusted optical density of each culture, pooled them at equal volume and inoculated 96 resource environments, with each condition replicated 8 times. Each culture was serially transferred for 16 days, with 1% of the culture transferred every 48 h. B | At each transfer, we measured OD 600 of each community. In addition, we conducted 16S rRNA sequencing of all communities at the end-point and measured growth traits of each of the dominant species ( A. caviae, P. chlororaphis, C. koseri ) in monoculture in all carbon levels in the absence of R2A. Alt text. Two-panel schematic representing the study set-up. Panel A describes parameters of the serial passaging experiment. Panel B explains analytical procedures of the community and single-species experiments.

    Article Snippet: R2A medium was prepared by dissolving R2A Broth powder (Neogen, UK) in M9 minimal medium.

    Techniques: Sequencing, Passaging

    A | Shannon diversity shown separately for each resource environment. The x- axis shows the total carbon level (doubling concentrations spaced evenly) and point color indicates resource complexity level. The figure contains 768 data points (8 replicates in 96 resources). B | Hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis). Points show the estimated peak height (hump statistic); error bars show 95 % bootstrap confidence intervals. C | Variance partitioning of Shannon diversity using nested linear models. Bars show unique R 2 contributions of total carbon level, resource complexity (% R2A carbon contribution), and their interaction. Alt text. Three-panel figure representing diversity measures, with panel A containing data on Shannon diversity, resolved for each resource environment, panel showing a hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis), and panel C showing variance partitioning of Shannon diversity using nested linear models.

    Journal: bioRxiv

    Article Title: Ecological tristability driven by total carbon availability over resource complexity in a synthetic microbial community

    doi: 10.64898/2026.02.26.708221

    Figure Lengend Snippet: A | Shannon diversity shown separately for each resource environment. The x- axis shows the total carbon level (doubling concentrations spaced evenly) and point color indicates resource complexity level. The figure contains 768 data points (8 replicates in 96 resources). B | Hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis). Points show the estimated peak height (hump statistic); error bars show 95 % bootstrap confidence intervals. C | Variance partitioning of Shannon diversity using nested linear models. Bars show unique R 2 contributions of total carbon level, resource complexity (% R2A carbon contribution), and their interaction. Alt text. Three-panel figure representing diversity measures, with panel A containing data on Shannon diversity, resolved for each resource environment, panel showing a hump metric for total carbon gradient ( y -axis) at each level of resource complexity ( x -axis), and panel C showing variance partitioning of Shannon diversity using nested linear models.

    Article Snippet: R2A medium was prepared by dissolving R2A Broth powder (Neogen, UK) in M9 minimal medium.

    Techniques: